EFFECTS OF HEPARIN ON THE LIPOPROTEINS IN HYPERLIPEMIA. AN ELECTROPHORETIC STUDY OF THE SERUM ALPHA AND BETA LIPOPROTEINS AFTER THEIR SEPARATION BY FRACTIONATION OF THE PLASMA PROTEINS OR ULTRACENTRIFUGAL FLOTATION 1

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Abstract
Electrophoretic analysis of the alpha and beta lipoproteins after their chemical separation (Cohn Method 10) revealed in both idiopathic and alimentary hyperlipemia as the result of an intravenous injection of heparin an increase in the electrophoretic mobility of both lipoproteins. The separated alpha lipoproteins moved faster than albumin and the separated beta lipoproteins moved with the speed of either alpha-2 or alpha-1 globulin. Electrophoretic analysis of the alpha and beta lipoproteins after their isolation by ultracentrifugal flotation showed an increase in their electrophoretic mobility following the injection of heparin. The alpha lipoproteins were present as 2 components in the post-heparin patterns obtained by paper electrophoresis. The pre-albumin component observed following the injection of heparin in the electrophoretic pattern of whole serum in diopathic as well as alimentary hyperlipemia represents the alpha lipoproteins. No evidence was found to support the recently proposed concept that the administration of heparin causes a transformation of beta lipoproteins into alpha lipoproteins.