THYROID EPITHELIUM IN TISSUE CULTURES1: OBSERVATIONS ON THE MORPHOLOGY AND FUNCTIONAL CAPACITIES OF EMBRYO CHICK THYROIDS1

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Abstract
Chick embryo thyroid fragments were cultivated in special tissue culture chambers designed primarily for cytological investigations of the living cell. Employing time-lapse techniques three secretory phenomena were observed. These were associated with (1) cytoplasmic droplets which were equivocally PAS positive and (2) cytoplasmic phase contrast green granules, the localization sites for I . Chicken whole egg ultrafiltrates stimulated the epithelial outgrowth to a marked degree but this was not apparent for the fibroblastoid stromal elements.