ELIMINATION OF INGESTED 32P-LABELLED E. COLI FROM RAT POLYMORPHONUCLEAR NEUTROPHILS (PMN)
- 15 August 2009
- journal article
- research article
- Published by Wiley in Acta Pathologica Microbiologica Scandinavica Section C Immunology
- Vol. 84C (2) , 100-104
- https://doi.org/10.1111/j.1699-0463.1976.tb00005.x
Abstract
A method for in vitro assay of the elimination of ingested 32P-labeled Escherichia coli from rat PMN monolayers was evaluated. The rate of expulsion of bacterial label from phagocytes into the extracellular medium ranged from 40-50% of the total uptake 180 min after termination of ingestion. Serum enhanced the cellular uptake of bacteria, but did not affect the rate of elimination. Disintegration of the phagocytes was not a problem.Keywords
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