Gene expression: chemical synthesis and molecular cloning of a bacteriophage T5 (T5P25) early promoter
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (17) , 5921-5940
- https://doi.org/10.1093/nar/11.17.5921
Abstract
A sixty base pair DNA duplex containing the nucleotide sequence of the bacteriophage T5 early (T5P25) promoter has been constructed using a combination of chemical synthesis and enzymatic methods. Subsequent to cloning into pBR322, the promoter has been demonstrated to be biologically active being capable of directing the efficient expression of genes under its control. This serves as a prototype for an approach to the study of the iri vivo structure-function relationships and efficiency of promoters.Keywords
This publication has 26 references indexed in Scilit:
- Compilation and analysis ofEscherichia colipromoter DNA sequencesNucleic Acids Research, 1983
- Contacts between Escherichia coli RNA polymerase and a lac operon promoter.Proceedings of the National Academy of Sciences, 1978
- Interaction of E. coli RNA polymerase with promotors of coliphage T5: the rates of complex formation and decay and their correlation with in vitro and in vivo transcriptional activity.1977
- Interaction of RNA Polymerase with Promoters from Bacteriophage fdEuropean Journal of Biochemistry, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresisBiochemistry, 1975
- Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structuresJournal of Molecular Biology, 1975
- Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd.Proceedings of the National Academy of Sciences, 1975
- [57] Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteriaPublished by Elsevier ,1975
- SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORMProceedings of the National Academy of Sciences, 1969