Quantification ofL-ascorbic acid and total ascorbic acid in fruits and spinach by capillary zone electrophoresis
- 4 May 2001
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 22 (8) , 1484-1488
- https://doi.org/10.1002/1522-2683(200105)22:8<1484::aid-elps1484>3.0.co;2-9
Abstract
A standard curve for the quantification of L‐ascorbic acid (L‐AA) by capillary zone electrophoresis (CZE) was established, and the quantification of ascorbic acid and total ascorbic acid in fruits (lemon, Sunkist, and pineapple) and spinach were performed using D‐isoascorbic acid (D‐IAA) as an internal standard. The minimum detection limits (MDLs) for L‐AA and D‐IAA were determined to be 1 and 2 νg/mL, respectively, at 265 nm. Dehydroascorbic acid (DHAA) in fruits and spinach was quantified in the presence of DL‐homocysteine. The recoveries for L‐AA in these juices were between 95 and 105%.Keywords
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