Stimulation of DNA‐mediated transformation by UV irradiation of recipient (mouse FM3A) cells
- 1 December 1984
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 121 (3) , 453-457
- https://doi.org/10.1002/jcp.1041210302
Abstract
DNA‐mediated transformation for thymidine autotrophs (Tk+) was stimulated severalfold when the recipient (mouse FM3A) (Tk−) cells were preirradiated by UV light. The effect was most prominently seen with the cells irradiated 12–16 hours prior to DNA addition. A similar stimulatory effect was observed when the cells were treated with novobiocin or mitomycin C. The effect, however, was blocked when cycloheximide was present during postirradiation period, suggesting that de novo protein synthesis is required for producing the effect. Most of the Tk+ transformants arised from the UV‐irradiated cells carried the donor DNA sequences in their chromosomes.Keywords
This publication has 16 references indexed in Scilit:
- The SOS regulatory system of Escherichia coliCell, 1982
- Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNASomatic Cell and Molecular Genetics, 1980
- Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.Proceedings of the National Academy of Sciences, 1979
- The transfer and stable integration of the HSV thymidine kinase gene into mouse cellsCell, 1978
- An Improved Technique for Obtaining Enhanced Infectivity with Herpes Simplex Virus Type 1 DNAJournal of General Virology, 1976
- Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cellsSomatic Cell and Molecular Genetics, 1976
- Model for regulation of Escherichia coli DNA repair functions.Proceedings of the National Academy of Sciences, 1975
- Herpes Simplex Virus as a Source of Thymidine Kinase for Thymidine Kinase-Deficient Mouse Cells: Suppression and Reactivation of the Viral EnzymeProceedings of the National Academy of Sciences, 1973
- Repair of damaged DNA in the absence of protein synthesis in mammalian cellsExperimental Cell Research, 1973
- Establishment of Cell Lines in vitro from a Mammary Ascites Tumor of Mouse and Biological Properties of the Established Lines in a Serum Containing MediumThe Tohoku Journal of Experimental Medicine, 1966