Influence of an Adequate Dietary Protein on the Immediate and Latent Effects of Stilbestrol

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Abstract
An 18% casein diet allowed the recovery of body weight loss produced by stilbestrol (0.1 mg x 21 days) during a recovery period of 0, 10, 20, 30 days, whereas male rats fed a protein free diet did not recover. Absence of dietary protein maintained a negative nitrogen balance induced by stilbestrol, but 18% dietary protein permitted return to positive nitrogen balance following hormone and was more pronounced in treated rats. Recovery on the protein diet resulted in the repletion of liver weight and total liver protein losses due to action of the hormone. Protein free diet allowed further marked depletion of liver protein and increased liver water concomitant with large increase in liver fat.