Pregnancy-Specific β1-Glycoprotein-Like Material in Human Cerebrospinal Fluid*

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Abstract
Human cerebrospinal fluid (CSF) from 34 unselected neurological patients was studied for pregnancy-specific/Ji-glycoprotein (SP1) activity because of the recent finding of SPl production by cultured glial cells. An organic central nervous system lesion was diagnosed in 9 patients, but not in the other 25. Low levels of SP1 immunoreactivity were found in CSF by RIA, and the adsorption of anti-SPl antiserum with concentrated CSF abolished the positive immunohistochemical staining of placental tissue obtained with the unadsorbed antiserum. By means of immunoadsorption using monoclonal anti-SPl antibodies, it was possible to isolate SP1 immunoreactive material from CSF and to demonstrate that it had the same electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as purified placental SP1. These results show that CSF contains SPl-like material that is closely related, if not identical, to placental SPl. The amount of SPl in CSF has no direct correlation to an organic central nervous system lesion or to abnormality of the CSF.
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