A Human‐Human Monoclonal Anti‐D by Direct Fusion with a Lymphoblastoid Line1
- 1 November 1986
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 51 (3) , 212-216
- https://doi.org/10.1111/j.1423-0410.1986.tb01955.x
Abstract
The human lymphoblastoid cell line W1-L2-729 HF2 has been fused with B cells from a plasmaphoresed anti-D donor immunized with D+ cells. A stable monoclonal antibody-producing cell line has been produced which yields culture supernatant of good titre without the need for concentration. The production and use of this reagent as an alternative Rh D typing reagent for use by saline and enzyme manual tests and automated tests in a Technicon 16C machine is discussed. Du red cells are detected in enzyme enhanced tests.This publication has 11 references indexed in Scilit:
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