AMNIOTIC-FLUID PHOSPHOLIPIDS MEASURED BY CONTINUOUS-DEVELOPMENT THIN-LAYER CHROMATOGRAPHY

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (3) , 403-405
Abstract
A 1 dimensional TLC system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine and phosphatidylethanolamine is described. Short-bed continuous development and high performance TLC are used. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.