Antibacterial Activity of Lividomycin Toward R Factor-Resistant Strains of Escherichia coli

Open Access
Publisher Website
Google Scholar
Abstract
Forty-eight R factors conferring resistance to both kanamycin (KM) and streptomycin (SM) were demonstrated from 1,270 Escherichia coli strains of clinical origin. Among these R factors isolated, 42 also conferred resistance to the new antibiotic lividomycin (LV). Extracts of E. coli ML1410 carrying one such factor (R M81 ) inactivated LV as well as KM and SM. But extracts of E. coli ML1410 carrying an R factor (R M82 or R M83 ) sensitive to LV could not inactivate LV. LV inactivation required both adenosinetriphosphate (ATP) and Mg 2+ . Inactivated LV was reactivable by alkaline phosphatase. Isotopic studies with labeled ATP and elemental analysis of the reaction product indicate that it is a monophosphorylated derivative of LV.