Further manifestations of abortive transformation of bhk 21 cells by polyoma virus

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Abstract
Abortive transformation of layer cultures of BHK 21 cells by polyoma virus has been studied using serum‐depleted medium. High doses of partially purified infectious virus, in the absence of serum, stimulate the majority of the cells to synthesize DNA while empty capsids do not. The onset of DNA synthesis occurs approximately 28 h after infection, and in medium containing 0.8% γ‐depleted serum, can be followed by one round of mitoses. A temporary loss of oriented arrangement of cells is also observed commencing 25 to 30 h after infection. Abortively transformed cells resemble stably transformed lines of BHK 21 cells in their requirements for the factors in serum operative in the control of growth.