Heat inactivation of human immunodeficiency virus in solutions of antithrombin III
- 1 February 1989
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 29 (2) , 148-152
- https://doi.org/10.1046/j.1537-2995.1989.29289146834.x
Abstract
Human immunodeficiency virus (HIV), isolated from cultures of infected peripheral blood lymphocytes, was added to solutions of highly purifed antithrombin III (AT-III), which was stabilized with 1 M citrate and 17 percent sucrose. The efficiency of heat inactivation of HIV in AT-III at 60.degree.C was compared with that of HIV in culture medium and followed for periods from 0 to at least 10 hours. The virus added, titer 105 by ID50, was inactivated as rapidly (less than 30 minutes) and efficienctly (completely) in the stabilized AT-III as in the culture medium. Virus was determined by direct measurement of the HIV-related reverse transcriptase activity and by quantiation of virus infectivity by ID50 assay.This publication has 17 references indexed in Scilit:
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