Sequence and centromere proximal location of a transformation enhancing fragmentanslfromAspergillus nidulans
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (22) , 9163-9176
- https://doi.org/10.1093/nar/15.22.9163
Abstract
The Asperqillus nidulans sequence ans1, previously known to enhance transformation frequencies of pyr4-based vectors, was shown to enhance the efficiency of arqB and trpC-based vectors. Increased efficiencies could be obtained by constructing vectors containing argB and ansl or by cotransforming selectable plasmids (containing argB, trpC or pyr4) with the non-selectable ansl sequence. The preponderance of evidence suggests that the mechanism of ansl activity does not involve homologous recombination events, in spite of the presence of multiple regions of homology in the A. nidulans genome. Genetic mapping localized ansl to the vicinity of the centromere of linkage group I. The nucleotide sequence of a 1.8 Kb functional subclone of ansl was determined and found to be highly A+T rich (81%).Keywords
This publication has 16 references indexed in Scilit:
- A novel sequence common to the centromere regions ofSchizosaccharomyces pombechromosomesNucleic Acids Research, 1987
- Analysis of centromeric DNA in the fission yeast Schizosaccharomyces pombe.Proceedings of the National Academy of Sciences, 1986
- Efficient cloning of genes of Neurospora crassaProceedings of the National Academy of Sciences, 1986
- Behaviour of recombinant plasmids in Aspergillus nidulans: structure and stabilityCurrent Genetics, 1986
- Transformation of Aspergillus nidulans with a cloned, oligomycin-resistant ATP synthase subunit 9 geneMolecular Genetics and Genomics, 1986
- Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD)Molecular Genetics and Genomics, 1986
- Functional selection and analysis of yeast centromeric DNACell, 1985
- Multiple, Tandem Plasmid Integration inSaccharomyces cerevisiaeMolecular and Cellular Biology, 1983
- Transformation of yeast.Proceedings of the National Academy of Sciences, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977