The State of Pancreatic and Serum Insulin: Results of Immunoassay
- 1 July 1965
- journal article
- research article
- Published by American Diabetes Association in Diabetes
- Vol. 14 (7) , 387-391
- https://doi.org/10.2337/diab.14.7.387
Abstract
Insulin fractionation of human and canine pancreata and sera has been studied by immunoassay. Several procedures designed to separate bound and free insulin have been utilized. Immunoassay has demonstrated pancreatic insulin to have a very low solubility in saline. However, a cation-bound fraction could not be demonstrated in either the pancreas or serum. These results suggest that cation-bound insulin-like activity demonstrated by rat diaphragm or adipose tissue assay does not represent pancreatic insulin.Keywords
This publication has 12 references indexed in Scilit:
- ANTIBODY-SUPPRESSIBLE AND NONSUPPRESSIBLE INSULIN-LIKE ACTIVITIES IN HUMAN SERUM AND THEIR PHYSIOLOGIC SIGNIFICANCE. AN INSULIN ASSAY WITH ADIPOSE TISSUE OF INCREASED PRECISION AND SPECIFICITY*Journal of Clinical Investigation, 1963
- The “Typical” and “Atypical” Forms of Serum InsulinDiabetes, 1963
- The Presence of Two Forms of Insulin in Normal Human SerumBiochemistry, 1963
- STUDIES ON STATE, TRANSPORT AND REGULATION OF INSULIN IN HUMAN BLOOD1962
- STUDIES ON THE STATE OF INSULIN IN BLOOD: DISSOCIATION OF PURIFIED HUMAN BLOOD INSULIN COMPLEX(ES) BY INCUBATION WITH ADIPOSE TISSUE EXTRACTSIN VITRO1Endocrinology, 1961
- IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MANJournal of Clinical Investigation, 1960
- Biological Activity of Blood Insulin Complexes Examined by Rat Diaphragm Tissue Assay.Experimental Biology and Medicine, 1960
- Preliminary Observations on State of Insulin in Human and Bovine Pancreas.Experimental Biology and Medicine, 1960
- INSULIN-LIKE ACTIVITY OF HUMAN PLASMA CONSTITUENTS .3. ELUTION OF INSULIN-LIKE ACTIVITY FROM CATIONIC EXCHANGE RESINS1958
- INSULIN-LIKE ACTIVITY OF HUMAN PLASMA CONSTITUENTS .2. BIOLOGIC ASSAY OF HUMAN PLASMA FRACTIONS FOR INSULIN-LIKE ACTIVITY1956