Location of genes controlling the D group of LMW glutenin subunits on chromosome 1D of bread wheat

Abstract
Summary: Over one hundred backcross-one progeny were analysed by two dimensional electrophoresis to locate the genes on the short arm of chromosome 1D which code for, or control, the D group of LMW glutenin subunits. Recombination between these genes andGlu-D 1, encoding HMW glutenin subunits, was frequent but none could be detected between the D subunit genes andGli-D1, coding for ω-gliadins, γ-gliadins and the B group of LMW glutenin subunits. These results are in contrast to those for chromosome 1 B because apparently homoeologous D subunit genes occur at a different position, being equidistant betweenGlu-B 1(homoeologous toGlu-D 1) andGli-B 1(homoeologous toGli-D 1). A range of 18 genetically-diverse wheat varieties, each containing one of two allelic groups of 1 D-encoded D subunits, were also analysed by two-dimensional electrophoresis. Consistent with the genetic analysis above, unbreakable linkages were found between these alleles and alleles ofGli-D 1. The results are discussed in relation to the evolution of the distribution of prolamin genes in the wheat genome.

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