The Sites of Action of Allopurinol in Crithidia fasciculata*

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Abstract
SYNOPSIS: Reversal of the growth inhibition of Crithidia fasciculata by allopurinol requires both a purine and a pyrimidine. Hypoxanthine is the most effective purine in the reversal. Cell‐free extracts were prepared which were capable of the decarboxylation of orotidine 5′‐phosphate. Other enzyme preparations carried out the phosphoribosylation of allopurinol. By the use of [4‐14C] orotidine 5′‐phosphate (enzymatically prepared), it was shown that allopurinol ribotide (enzymatically prepared), but not the free base, inhibits orotidine 5′‐phosphate decarboxylase.