INTERCELLULAR TRANSFER OF GAMMA-1 AND GAMMA-2 FORSSMAN HEMOLYSINS

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Abstract
The percent net intercellular transfer of hemolysin from red cell to red cell was studied during the initial and anamnestic hemolysin responses in rabbits given repeated injections of 1.6 X 109 heated sheep red blood cell stromata (SStr) 3 X a week for a month, or given similar repeated or single injections of heated human A red blood cell stromata (AStr). Intercellular transfer was also studied in some of the anti-SStr serums after electrophoretic separation. In general, during corresponding immunizations, changes in the hemolysin response and in the percent net transfer were greatest with multiple injections of SStr, were intermediate with multiple injections of AStr, and were least with single injections of AStr. Moreover, with a given antigen, the changes were more marked in the initial immunization than in the anamnestic responses. Thus, with both antigens, hemolysin titer was, in general, reciprocally related to percent transfer during the initial immunization, but transfer sometimes remained high at peak titer during the 2d and especially during the 3d immunization. This result, with respect to transfer in the rabbits immunized with SStr, was associated with a progressive increase in the gamma2 hemolysin with a high transfer rate and a decrease in the gamma1 hemolysin with a low transfer rate. Inasmuch as little or no gamma2 hemolysin has been found in various investigators in response to AStr, the high rates of transfer in the anamnestic responses to AStr were possible associated with a relative increase in normal hemolysins as production of immune hemolysins decreased. Transfer was ascertained in terms of the number of 50% units of hemolysin needed to be absorbed on unlabeled red cells to give the net transfer of one 50% unit of hemolysin to unsensitized Cr51-labeled red cells. The slope of the von Krogh graphs in the tests was usually 0.4 for the gamma1 hemolysin and 0.2 for the gamma2 hemolysin.
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