EFFECT OF ACTINOMYCIN D ON RNA SYNTHESIS AND ANTIBODY FORMATION IN THE ANAMNESTIC RESPONSE IN VITRO

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Abstract
Antibody synthesis in anamnestic lymphoid cells, measured by incorporation of Leucine-Cl4 into specific antibody, was inhibited at moderate concentrations of actinomycin-D. There was a marked inhibition of synthesis of ribonucleic-acid (RNA) as measured by incorporation of H3-cytidine monophosphate. At low concentrations of actinomycin-D, antibody synthesis was unaffected or even increased; while RNA synthesis continued to be inhibited. Messenger RNA for antibody synthesis, either because it is relatively stable or present in excess, does not become a limiting factor until its synthesis is maximally inhibited. Puromycin, an inhibitor of amino-acid coupling, abolished antibody synthesis in low concentration. 6-Mercaptopurine even at a high concentration had no effect on the synthesis of antibody or RNA. Antibody synthesis probably follows pathways similar to those utilized for the formation of other types of proteins.

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