37—THE SITES OF REACTION OF PHENYL ISOCYANATE WITH WOOL

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Abstract
An investigation is described in which some of the sites of reaction of phenyl isocyanate with wool in dimethylformamide solution were identified by analysis of enzyme hydrolysates of the treated wool. These hydrolysates were prepared by oxidation of the wool with performic acid, followed by digestion with three proteolytic enzymes. This procedure releases amino-acid derivatives, which would be converted to the parent amino acids by conventional acid hydrolysis. In one case, the analysis was facilitated by the use of radioactively labelled phenyl isocyanate. Four amino-acid derivatives, arising from the reaction of phenyl isocyanate with the side chains of lysine, serine, threonine, and glutamic acid, were found in the hydrolysates. Their identities were confirmed by comparison with authentic samples, whose synthesis is described.

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