Metabolic properties of histones from rat liver and thymus gland

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Abstract
The incorporation of C14-labelled amino acids into acid-extractable proteins from rat-liver and -thymus nuclei confirmed the existence of a protein component with a higher uptake than that into the major histone components. This rapidly labelled component appeared to contain the thiol groups detectable in the acid extracts. Histone f1 contained 1 mol. of serine phosphate/mol. of mol.wt. of 42000-43000. Phosphate was present in other components of the 50 mM-hydrochloric acid extract from liver and thymus nuclei, and was probably associated with the thiol-containing component. The difference in amino acid uptake into the histones of diffuse and dense chromatin was confirmed. Dense chromatin was found to have a higher proportion of disulphide than did diffuse chromatin.