Lipolysis of Synthetic Triglycerides and Milk Fat by a Lipase Concentrate from Milk

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Abstract
The behavior of a milk lipase concentrate (Shahani) on simple triglycerides, diacid triglycerides and milk fat was studied. In simple triglyceride substrate systems the concentrate was relatively more active on short-chain and unsaturated fatty-acids than on long-chain fatty-acids. When the substrate was a triglyceride with palmitic acid in one primary position and caproic acid or oleic acid in the other, the lipase concentrate preferentially lipolyzed the external positions with no specificity for short-chain or unsaturated acids. The lipase concentrate resembles B-esterase and crude milk lipase(s) in its mode of action.