Absence of Fatty Acid Specificity During Lipolysis of Some Synthetic Triglycerides by B-Esterase Preparations from Milk

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Abstract
Glyceryl 1-oleate 2,3-dicaproate (OCC) and glyceryl 1-palmitate 2,3-dibutyrate (PBB) emulsified into skimmilk were lipolyzed by B-esterase preparations for varying periods of time. The products of lipolysis were isolated by column and thin-layer chromatography. The fatty acid compositions of the isolated fractions were determined by gas-liquid chromatography. There was little or no specificity for caproate or butyrate during lipolysis periods of up to 1 hr. When PBB was lipolyzed for 24 hrs at 4C, some apparent specificity for butyrate was indicated.
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