Elution of Exocellular Enzymes from Saccharomyces fragilis and Saccharomyces cerevisiae

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Abstract
Weimberg, Ralph (Northern Regional Research Laboratory, Peoria, Ill.), and William L. Orton . Elution of exocellular enzymes from Saccharomyces fragilis and Saccharomyces cerevisiae . J. Bacteriol. 91: 1–13. 1966.—Invertase and acid phosphatase are repressible exocellular enzymes in Saccharomyces fragilis and S. cerevisiae . The conditions for eluting these enzymes from both organisms were compared. Either KCl or β-mercaptoethanol eluted the enzymes from S. fragilis , and the amounts eluted varied quantitatively according to the physiological age of the organism. In addition to eluting enzymatic activity from the cells, these reagents also caused a large increase in the amount of activity that remained associated with the cells of S. fragilis . Invertase and acid phosphatase were not removed from cells of S. cerevisiae by KCl or β-mercaptoethanol. These enzymes were separated from S. cerevisiae cells only when there was some degree of cell-wall digestion by snail gut fluid.

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