The phospholipids of pigeon and ox skeletal muscle

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Abstract
The phospholipids of pigeon breast muscle and ox longissimus dorsi muscle have been investigated. Considerable amounts of proteolipid were present in the lipids extracted from pigeon breast muscle by chloroform-methanolboth at the boiling point of the solvent and at 0[degree]. The lipids extracted from ox longissimus dorsi muscle by chloroform-methanol at 0[degree] contained very little proteolipid. The major ninhydrin-positive component of the phospholipids of both muscles, which was eluted from silicic acid by a concentration of methanol in chloroform similar to that which elutes phosphatidylethanol-amine, was different from this compound. Mild alkaline hydroly-sis liberated from it a water-soluble compound containing phosphorus and reacting with ninhydrin, which behaved differently from glycerylphosphorylethanolamine on an ion-exchange column and separated from glycerylphosphorylethanolamine on paper chromatog-raphy in one solvent system.