Functional regulation of metabotropic glutamate receptor type 1c: a role for phosphorylation in the desensitization of the receptor
Open Access
- 30 November 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 462 (3) , 278-282
- https://doi.org/10.1016/s0014-5793(99)01547-1
Abstract
The phosphorylation and desensitization of metabotropic glutamate receptor type 1c in response to agonist and phorbol esters has been studied. Specific immunoprecipitation of mGluR1c from cells treated with agonist or PMA showed a time‐dependent increase in the phosphorylation of a membrane protein with the same molecular weight as the dimeric form of the receptor. Measurements of inositol phosphate production showed a rapid functional desensitization of about 90% after agonist treatment, whereas treatment with PMA caused only a 30% loss in the same time. The extent of receptor phosphorylation following the different treatments paralleled the desensitization of the receptor. These results strongly suggest that phosphorylation of the dimeric form of mGluR1c, as a functionally active form, may play a role in its rapid desensitization.Keywords
This publication has 30 references indexed in Scilit:
- Characterization of the Dimerization of Metabotropic Glutamate Receptors Using an N‐Terminal Truncation of mGluR1αJournal of Neurochemistry, 1999
- Cell surface expression of the metabotropic glutamate receptor type 1α is regulated by the C‐terminal tailFEBS Letters, 1999
- Role of G Protein–Coupled Receptor Kinase 2 and Arrestins in β-Adrenergic Receptor InternalizationTrends in Cardiovascular Medicine, 1998
- Metabotropic Glutamate Receptor 5 Is a Disulfide-linked DimerPublished by Elsevier ,1996
- Effect of Different G Protein-coupled Receptor Kinases on Phosphorylation and Desensitization of the α1B-Adrenergic ReceptorJournal of Biological Chemistry, 1996
- Immunological identification of A1 adenosine receptors in brain cortexJournal of Neuroscience Research, 1995
- Rapid agonist mediated phosphorylation of the metabotropic glutamate receptor 1α by protein kinase C in permanently transfected BHK cellsFEBS Letters, 1995
- Desensitization of Metabotropic Glutamate Receptors in Neuronal CulturesJournal of Neurochemistry, 1991
- Muscarinic and Quisqualate Receptor‐Induced Phosphoinositide Hydrolysis in Primary Cultures of Striatal and Hippocampal Neurons. Evidence for Differential Mechanisms of ActivationJournal of Neurochemistry, 1989
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970