IMMUNOHISTOCHEMISTRY OF SECRETORY PROTEINS IN THE BULL SEMINAL-VESICLE

  • 1 February 1987
    • journal article
    • research article
    • Vol. 150, 43-48
Abstract
Antibodies against ribonuclease, bull seminal proteinase inhibitor and seminal antimicrobial protein were used to identify the secretion sites of the respective proteins within the genital tract of the bull. Consistent positive immunoreactions were achieved with the former two antisera in the seminal vesicle epithelium, while only weak or dispersed immunoreactions were found in the epididymis, ductus deferens or the prostate. Comparison of serial sections of bull seminal vesicle stained with different antisera gave incongruous distribution patterns of positively reacting cells, possibly a processing artifact. The immunoreaction with the antibody against seminal antimicrobial protein was weak in the seminal vesicle epithelium. Definite evidence for the secretion of this protein by the seminal vesicle will be achieved only by in situ hydridization techniques.