Atomic Force Microscopy Of Uncoated Plasmid DNA: Nanometer Resolution with only Nanogram Amounts of Sample
- 1 December 1992
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 10 (3) , 607-617
- https://doi.org/10.1080/07391102.1992.10508671
Abstract
Reproducible, high-contrast, nanometer-resolution AFM images of uncoated plasmid DNA can be obtained with nanogram quantities of DNA with the help of two advances in sample preparation: (1) Heating a DNA solution at 35 °C for 10 to 20 minutes before deposition on mica helps separate and spread the DNA and (2) Using 5 microliter drops of the heated DNA solution in the concentration range of 2 to 10 nanogram/microliter in contact with a specially prepared mica surface for 5 to 10 minutes gives optimal coverage with only nanograms of DNA.Keywords
This publication has 16 references indexed in Scilit:
- DNA looping and Sp1 multimer links: a mechanism for transcriptional synergism and enhancement.Proceedings of the National Academy of Sciences, 1991
- Direct observation of B-form and Z-form DNA by scanning tunneling microscopyJournal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 1991
- Atomic Force MicroscopyPhysics Today, 1990
- Atomic-scale imaging of DNA using scanning tunnelling microscopyNature, 1990
- Images of the DNA Double Helix in WaterScience, 1989
- Scanning Tunneling Microscopy of Uncoated recA-DNA ComplexesScience, 1989
- Direct Observation of Native DNA Structures with the Scanning Tunneling MicroscopeScience, 1989
- Atomic Force MicroscopePhysical Review Letters, 1986
- Principles of Nucleic Acid StructurePublished by Springer Nature ,1984
- 7 × 7 Reconstruction on Si(111) Resolved in Real SpacePhysical Review Letters, 1983