SOME ASPECTS OF THE CHARACTERIZATION OF PREGNANT MARE SERUM GONADOTROPHIN

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Abstract
Two different preparations of pregnant mare serum gonadotropins (PMSG) were purified by means of zone electrophoresis on starch. A separation of the 2 biological activities was not possible but the resolution of a product possessing a minimal specific activity of 30,000 IU/mg was achieved and its isoelectric point was located at pH 2.4. It was found that the follicle-stimulating and interstitial cell-stimulating activities of the starting preparations were retained in similar proportions throughout the purification, and no evidence was obtained from the electrophoretic studies or from the studies with periodate-treated hormone that would suggest that the 2 activities are not inherent properties of the PMSG molecule.