Abstract
Enzymatic sulfation of bile salts was identified in cytosol prepared from human adrenal tissue. Unconjugated lithocholate was sulfate at a slightly greater rate than glycolithocholate. Chenodeoxycholate and deoxycholate were sulfated at the 7- and 12-OH positions, respectively, but no disulfate formation from these bile salts was found. The physiological implications of these results are discussed.