STUDIES ON ESTER SULPHATES

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Abstract
A survey of the distribution of phenol and steroid sulfokinases in various adult human. tissues was undertaken by the use of 35s-labelled 3''-phosphoadenosine-5''-phosphosulfate (PAPS). The following healthy tissues were studied: adrenals, jejunal mucosa, kidneys, liver, ovaries and spleen. In addition, some determinations were made on pathological tissues, i. e. cirrhotic liver, an adrenal adenoma, hypernephromas, nephritic renal tissues and various thyroid adenomas. Phenol sulfokinase was present in all the tissues studied. With 1 exception, the various steroid sulfokinases (dehydroepiandrosterone [DHA] androsterone, aetiocholan-3a-ol-17-one, testosterone, pregnan-3[alpha]-ol-20-one, pregnan-3[beta]-ol-20-one, 11-deoxycorticosterone [DOC] and estrone sulfokinase) were detectable in3 organs only (liver, adrenals and jejunal mucosa). The exception was the presence of estrone sulfokinase in a thyroid adenoma of "fetal" type. In the pathological tissues, the level of the enzyme activities did not deviate significantly from that in normal tissues. The pH optima of some sulfokinases were estimated and found to be 5.8 for DHA sulfokinase, 5.5 for androsterone sulfokinase and 6.8 for phenol sulfokinase in the liver preparations. In the adrenal preparations the pH optimum for DHA and DOC sulfokinase was 6.3 and that for androsterone sulfokinase 5.9.