3 DAY PLEURAL INFLAMMATION - A NEW MODEL TO DETECT DRUG EFFECTS ON MACROPHAGE ACCUMULATION

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 215  (3) , 588-595
Abstract
Mononuclear cell accumulation is of major importance in maintaining chronic inflammatory conditions. To model this phenomenon, 0.3 ml of a 1% carrageenan solution was injected into the pleural cavity of rats; at various times thereafter peripheral blood and pleural exudate samples were collected. After carrageenan injection (72 h), 82.3 .+-. 3.7 .times. 106 cells (no. = 6; mean .+-. SE) were present in the pleural cavity; over 80% of the cells were macrophages as determined by morphologic and histochemical criteria. Animals treated with dexamethasone had a significantly reduced number of pleural macrophages. Animals treated with the nonsteroidal anti-inflammatory agents, naproxen and indomethacin, had an elevated intrapleural macrophage content. The number of intrapleural cells was not affected by the antirheumatic agents levamisole, d- and dl-penicillamine or gold sodium thiomalate. Animals treated with tilorone, dapsone, hydroxychloroquine, phenylmethane-sulfonyl fluoride and 1,10 phenanthroline had a reduced pleural cell count.