Demonstration of high molecular weight poly (adenosine diphosphate ribose)

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Abstract
An electrophoretic system was established that resolves poly-(adenosine diphosphate ribose), enzymatically synthesized polymer from NAD + , by size difference of one residue on polyacrylamide gel. The existence of a polymer of at least 65 residues was demonstrated by band counting in this system. The polymer showed a heterogeneous size distribution on the electrophoregram, and the molecular weight of the largest polymer was deduced to be more than 4.5 × 10 5 daltons. The discrepancy between the size, estimated by electrophoresis, and the chain length, determined by the ratio of total radioactivity to that derived from the terminus, suggests that the polymer has a branched structure.