Targeting frequency for deletion vectors in embryonic stem cells.
- 1 April 1994
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 14 (4) , 2404-2410
- https://doi.org/10.1128/mcb.14.4.2404
Abstract
We analyzed the gene targeting frequencies and recombination products generated by a series of replacement deletion vectors which target the hprt (hypoxanthine phosphoribosyltransferase) locus in mouse embryonic stem cells. We found that the targeting frequency of a 19.2-kb deletion was comparable to that of a 3-kb deletion or a conventional replacement event in which a 1.7-kb fragment was inserted into the locus. We also observed different integration patterns for these deletion vectors. A result of this finding is that a wide range of genomic deletions in embryonic stem cells is feasible.Keywords
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