Staining Processed Radioautographs
- 1 January 1961
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 36 (5) , 313-317
- https://doi.org/10.3109/10520296109113298
Abstract
Integrated radioautographs obtained by coating with melted nuclear emulsion (Eastman''s NTB3) are serially processed and stained at 4[degree]C. Kingsley''s mixture of methylene blue-azure A at pH 6.9 is followed by 0.05% NaHSOg, which destains the emulsion. Basic fuchsin, 0.05% aqueous, is used as counterstain, followed by dehydration in absolute ethanol, clearing in cedarwood oil and mounting with Canada balsam. Basic fuchsin alone produces an oftentimes satisfactory monochromatic background.Keywords
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