Effect of Malonate and Antimycin A on Renal Tubular Transport of p-Aminohippurate.

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Abstract
Administration of sodium malonate to rats reduces the capacity of cortical slices prepared from the kidneys of such animals to accumulate p-aminohippuric acid (PAH). The degree of inhibition is proportional to the size of the dose, a linear relationship being obtained with intravenous doses from 0.6 to 1.0 g/kg. This inhibitory effect is reversible, maximum inhibition of PAH transport occurring approximately 1 hour after administration of 1.0 g/kg of malonate followed by complete recovery after another hour. Addition of antimycin A, another inhibitor of succinate metabolism, to a medium containing renal cortical slices, reduces their capacity to accumulate PAH. A similar impairment of this mechanism occurs when the agent is administered intravenously or intraperitoneally to the intact rat. These findings correlated with the results of other investigators, strongly suggest succinate oxidation can provide an in vivo source of energy for the renal tubular transport of PAH.