Isolation and Characterization of Canine Distemper Virus-Specific RNA

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Abstract
Ten species of virus-specific RNA were detected in Vero cells infected with the FXNO strain of canine distemper virus (CDV). The largest RNA was the genome-sized RNA and the 9 smaller species were polyadenylated RNA. Similar results were obtained for 9 other strains of CDV. The MW of these 10 RNA were 4.61 .times. 106, 2.46 .times. 106, 1.52 .times. 106, 1.32 .times. 10.6, 1.19 .times. 106, 1.07 .times. 106, 0.77 .times. 106, 0.65 .times. 106, 0.58 .times. 106 and 0.48 .times. 106. By in vitro translation of the polyadenylated RNA in a rabbit reticulocyte lysate system, 3 different proteins which probably correspond to H, NP and M were synthesized from the fraction containing RNA, 7, 8, 9 and 10.