PRIMARY STRUCTURE OF THE NEUROSPORA PLASMA-MEMBRANE H+-ATPASE DEDUCED FROM THE GENE SEQUENCE - HOMOLOGY TO NA+/K+-ATPASES, CA2+-ATPASES, AND K+-ATPASES
- 15 November 1986
- journal article
- research article
- Vol. 261 (32) , 4896-4901
Abstract
The gene for the Neurospora crassa plasma membrane H+-ATPase has been cloned and sequenced. The gene encodes for a protein of 920 amino acids with a molecular weight of 100,002. The coding region is interrupted by four introns: three near the amino terminus and one near the carboxyl terminus. The deduced amino acid sequence of the N. crassa plasma membrane H+-ATPase exhibits 75% homology to the amono acid sequence of the Saccharomyces cerevisiae plasma membrane H+-ATPase. Also, an amino acid comparison with the Na+/K+-ATPase from sheep kidney, Ca2+-ATPase from rabbit muscle, and K+-ATPase from Escherichia coli reveals that certain regions are highly conserved functions which are common to the various cation-motive ATPases. This observation suggests that the phosphorylatable, cation-motive ATPases may function via a similar energy transduction mechanism.This publication has 28 references indexed in Scilit:
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