DETECTION, ELIMINATION, AND PREVENTION OF CONTAMINATION OF CELL CULTURES WITH PLEUROPNEUMONIALIKE ORGANISMS

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Abstract
Procedures have been given for the detection, elimination, and possible prevention of cell culture contamination by pleuropneumonia-like organisms (PPLO) or closely related microorganisms. PPLO were isolated on serum agar directly and after enrichment by rapid serial transfers in serum broth. Identification was made on the basis of colony morphology and microscopic appearance of the organisms. Permanent elimination of the contaminants was achieved by treating cultures with doses of Aureomycin which were toxic for the cells. The procedures recommended to prevent PPLO (re-)contamination include the use of filter pads of unusually small porosity, and the maintenance of cell cultures in antibiotic-free media.