A Raffinose-Sucrose Transgalactosidase from Wheat Germ

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Abstract
A transgalactosidase was isolated from extracts of wheat germ which transfers a-galactosyl units from raffinose to a molecule of sucrose, thus regenerating raffinose. Melibiose, stachyose, lactose, galactose 1-phosphate, alone or plus adenosine triphosphate or uridine triphos-phate, uridine diphosphate galactose, and adenosine diphosphate galactose are inactive as galactose donors. Fructose, glucose, inositol, or galactinol are ineffective as acceptors of the galactosyl moiety of raffinose. The apparent specificity of the reaction seems to be different from that of the a-transgalactosidase action observed with a-galacto-side galactohydrolase.