Direct and Indirect Homogeneous Enzyme Immunoassay of Benzodiazepines in Biological Fluids and Tissues

Abstract
The evaluation of a new homogeneous enzyme immunoassay (EMIT) for benzodiazepines is described. The assay, although developed for direct analysis in serum, has been used to detect benzodiazepines in other body fluids and tissues; this is accomplished through two processes. The first consists of a previously reported technique of extraction and reconstitution of the original specimen. The second consists of dilution of the original specimen. The assay is compared to a gas chromatographic procedure.