Absence of Effect of Angiotensin II on Adenylate Cyclase in Rat Proximal Renal Tubular Basolateral Membranes

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Abstract
3H-Angiostensin II binding was studied in rat renal proximal tubular basolateral membranes. The characteristics of binding were found to be consistent with a receptor for angiotensin II at this site. Effects of angiotensin II on cAMP production were studied in the basolateral membranes. Angiotensin II had no effect onthe time course of basal or parathyroid hormone stimulated cAMP production in basolateral membranes. Angiotensin II did not exhibit a concentration-dependent effect on cAMP production in basolateral membranes, either alone or in the presence of parathyroid hormone. The concentration-response curves of cAMP production in response to parathyroid hormone in basolateral membranes were similar to the presence or in the absence of angiotensin II. We conclude that adenylate cyclase production is not linked to the receptor for angiotensin II in rat renal proximal tubular basolateral membranes.