A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II
- 1 January 1992
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 355 (6359) , 464-467
- https://doi.org/10.1038/355464a0
Abstract
RAP30/74 (also known as TFIIF (refs 1, 2), βΓ (ref. 3) and FC (ref. 4)) is one of several general factors required for initiation by RNA polymerase II (reviewed in refs 5–7). The small RAP30 subunit of RAP30/74 binds directly to polymerase and appears structurally and functionally homologous to bacterial sigma factors in their RNA polymerase-binding region8–10. RAP30/74 or recombinant RAP30 suppresses nonspecific binding of RNA polymerase II to DNA (refs 10,11) and is required for RNA polymerase II to assemble stably into a preinitiation complex containing promoter DNA and the general factors TFIID, TFIIA and TFIIB (refs 2,12, 13); both RAP30 and RAP74 are physical components of the preinitiation complex2. A complementary DNA encoding human RAP30 has been isolated8, and here we report the isolation of a cDNA encoding human RAP74. RAP30 and RAP74 produced in Escherichia coli can be used in place of natural human RAP30/74 to direct accurate transcription initiation by RNA polymerase II In vitro.Keywords
This publication has 21 references indexed in Scilit:
- The small subunit of transcription factor IIF recruits RNA polymerase II into the preinitiation complex.Proceedings of the National Academy of Sciences, 1991
- RNA polymerase II-associated proteins are required for a DNA conformation change in the transcription initiation complex.Proceedings of the National Academy of Sciences, 1991
- Related RNA Polymerase-binding Regions in Human RAP30/74 And Escherichia coli σ70Science, 1991
- Mechanism of promoter selection by RNA polymerase II: mammalian transcription factors alpha and beta gamma promote entry of polymerase into the preinitiation complex.Proceedings of the National Academy of Sciences, 1991
- An RNA Polymerase II Transcription Factor Shares Functional Properties with Escherichia coli σ 70Science, 1990
- RNA POLYMERASE B (II) AND GENERAL TRANSCRIPTION FACTORSAnnual Review of Biochemistry, 1990
- A heteromeric transcription factor required for mammalian RNA polymerase IINucleic Acids Research, 1990
- Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase IINature, 1989
- Initiation of transcription by RNA polymerase IIBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1989
- Promoter specificity and modulation of RNA polymerase II transcriptionThe FASEB Journal, 1989