Fertility of Frozen-Thawed Boar Semen

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Abstract
Farrowing results of 6 fertility tests were presented. Semen was processed by removal of seminal plasma and addition of glycerol at 30.degree. C before (method A) or after (method B) stepwise cooling to 5.degree. C, and by the Beltsville method (C). Sperm concentrations at freezing were 0.9 .times. 109/ml (A and B) and 0.6 .times. 109/ml (C). Farrowing rates for semen processed by methods A and B and pellet-frozen in tris-fructose-EDTA (TFE) diluent were 11/16 and 9/16 (farrowed/inseminated, mean 62.5%), and 10/16 for both single and double inseminations (10 .times. 109 and 20 .times. 109 total motile sperm, respectively). When equal numbers of motile spermatozoa from semen processed by method A were deposited by single and double inseminations, farrowing rates for sperm doses of 1.25 .times. 109, 2.50 .times. 109 and 5.00 .times. 109 were 7/16, 7/16 and 12/17, respectively. The number of inseminations within sperm dose had no effect on fertility. Pregnancies for semen processed by method A and frozen as pellets (0.25 ml) or in plastic bottles (30 ml) were 6/16 and 2/16, and after single insemination of 10 .times. 109 motile spermatozoa by non-fractionated and fractionated methods 6/16 and 2/16, respectively. Farrowing for semen pellet-frozen by method C in Beltsville F5 (BF5) extender was 5/10 after double insemination with 6 .times. 109 total (motile and non-motile) spermatozoa/dose. Fertility rates for semen processed by method A, pellet-frozen in TFE or Beltsville F3 (BF3) diluents and stored for 1 yr were 4/10 and 3/10, respectively (mean 35%), after single insemination of 10 .times. 109 motile spermatozoa. The mean litter size for the 89 sows farrowing was 9.4 .+-. 2.9.

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