Molecular cytogenetic analysis of the breakpoint region at 6q21–22 in T‐cell lymphoma/leukemia cell lines

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Abstract
Chromosome band 6q21 is reported to be one of the most frequent target regions in T‐cell lymphoma for both translocations and deletions. To explore whether the breakpoint clustering in T‐cell malignancy indicates the presence of a common breakpoint region in 6q, we employed fluorescence in situ hybridization analysis using various YAC, BAC, and PAC clones aligned at 6q21–22. We identified two T‐cell lymphoma/leukemia cell lines with different differentiation stages that had breakpoints within the same novel gene, TCBA1 (T‐cell lymphoma breakpoint associated target 1). In a T‐cell lymphoblastic lymphoma cell line, HT‐1, the TCBA1 fused to SUSP1 (SUMO‐1‐specific protease), creating a SUSP1‐TCBA1 chimeric gene. However, in an adult T‐cell leukemia cell line, ATN‐1, no chimeric gene was detected, although aberrant TCBA1 transcripts were produced. We conclude that TCBA1 is a possible target gene for T‐cell lineage‐specific chromosome aberrations at 6q21.