Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes
- 1 April 1990
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 172 (4) , 2178-2180
- https://doi.org/10.1128/jb.172.4.2178-2180.1990
Abstract
The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo titration of raf repressor, consists of two nearly identical 18-base-pair palindromes that flank the -35 box of the raf promoter.This publication has 19 references indexed in Scilit:
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