Purification and partial characterization of the gastric ulcer inhibitory substance from culture filtrate of Bacillus subtilis H.

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Abstract
The inhibitory substance against gastric ulceration in pylorus-ligated rats was purified through extraction with 5% acetic acid-50% ethanol, fractionation with ethanol, isoelectric precipitation, DEAE-cellulose column chromatography and gell filtration on Sephadex G-100 column from isoelectric precipitate if the 72 h culture filtrate of Bacillus subtilis H IAM 1521. It was named gastric ulcer inhibitory substance (GUIS). This substance reduced ulceration in pylorus-ligated rats by 84.2% at the dose of 5.0 mg/kg. It also significantly repressed aspirin-induced gastric lesions under pylorus ligation at the same dose, but its activity was weak in stress-induced ulceration. It markedly decreased gastric juice volume, acidity and peptic activity in pylorus-ligated rats when administered i.p. at 5.0 mg/kg. This substance was a glycoprotein which showed homogeneous patterns in various kinds of electrophoresis, and its isoelectric point was pH 4.5.