Interconversion between Monomer and Dimer of the 7S Globulin of Soybean Seed
- 1 January 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 42 (1) , 25-30
- https://doi.org/10.1080/00021369.1978.10862917
Abstract
The 7S globulin of soybean seed was purified on concanavalin A-Sepharose 4B. It exists as monomers at a high ionic strength or at a low pH, whereas it associates to form dimers under the opposite conditions. The sedimentation coefficient of the monomer is 5.6S, and that of the dimer 10S, at a protein concentration of 0.16%. Since the interconversion between the monomer and the dimer proceeds fairly rapidly, the ultracentrifugal pattern of the mixture shows a single peak. The apparent sedimentation coefficient of the mixture successively changes between 5.6S and 10S, corresponding to the ratio of the amounts of the monomer and the dimer. The monomer reversibly dissociates into subunits with 5 m urea or 0.01 n HC1.This publication has 2 references indexed in Scilit:
- Ultracentrifugal investigation of the effect of neutral salts on the extraction of soybean proteinsArchives of Biochemistry and Biophysics, 1956
- Ultracentrifuge studies on soya bean proteinBiochimica et Biophysica Acta, 1955