Determination of Zinc in Erythrocytes, Granulocytes and Serum by Flame Atomic Absorption Spectrophotometry

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Abstract
Zinc was determined in granulocytes, erythrocytes and serum by flame atomic absorption spectrophotometry. Using Teflon sampling cup, the sample volume for a single granulocyte zinc determination was reduced to 100 μl. Zinc in serum and erythrocytes was measured by aspirating the sample at a rate of 5 ml/min. The total volume of blood used was 23-28 ml. The intra-assay coefficient of variation of the method for granulocytes was 0.01 and of the method for serum and erythrocytes 0.018. The corresponding inter-assay coefficient of variation was 0.037 and 0.03, respectively. Recovery of zinc determined by the method of aspirating the small volume (100 μl) was 103% and by the method of aspirating 5 ml/min 97%. The coefficient of correlation between the two methods was r = 0.9968.