Amino-acid sequence of the β-subunit of the (Na+ + K+)ATPase deduced from a cDNA
- 1 May 1986
- journal article
- Published by Springer Nature in Nature
- Vol. 321 (6068) , 429-431
- https://doi.org/10.1038/321429a0
Abstract
The sodium/potassium-dependent ATPase [(Na+ + K+)ATPase], which establishes and maintains the Na+ and K+ gradients across the plasma membrane of animal cells, consists of two subunits, alpha and beta. Complementary DNA clones encoding the catalytic (alpha) subunit of sheep kidney and Torpedo californica electroplax enzymes have previously been isolated and characterized. However, there is little information concerning the primary structure of the beta-subunit, a glycoprotein of unknown function and relative molecular mass (Mr) approximately 55,000 (ref. 3). Here we describe the isolation and characterization of a cDNA clone containing the entire coding region of the beta-subunit of the sheep kidney (Na+ + K+)ATPase. We also discuss structural aspects of the protein and present evidence for a possible evolutionary relationship with the KdpC subunit of the Escherichia coli K+-ATPase.Keywords
This publication has 24 references indexed in Scilit:
- Papain fragmentation of the sodium-potassium ATPase .beta. subunit reveals multiple membrane-bound domainsBiochemistry, 1985
- Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNANature, 1985
- Primary structure of the α-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequenceNature, 1985
- Sequence homology between two membrane transport ATPases, the Kdp-ATPase of Escherichia coli and the Ca2+-ATPase of sarcoplasmic reticulum.Proceedings of the National Academy of Sciences, 1984
- Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAsNucleic Acids Research, 1984
- Labeling of intramembrane segments of the α-subunit and β-subunit of pure membrane-bound (Na+ + K+)-ATPase with 3-trifluoromethyl-3-(m-[125I]iodophenyl)diazirineBiochimica et Biophysica Acta (BBA) - Biomembranes, 1983
- Hydrophobic labeling of (sodium, potassium)-ATPase: further evidence that the .beta. subunit is embedded in the membrane bilayerBiochemistry, 1983
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Immunochemical evidence for a transmembrane orientation of both the (sodium(1+), potassium(1+) ion-activated)-ATPase subunitsBiochemistry, 1981
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976